Cas 200 Alfa Laval New Version
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SAFETY DATA SHEET Document number First issued Revision date Revision Issued by Page 53708601-ENG-4 2004-03-15 2011-12-20 3 Christine Diedrich MOLYKOTE(R) G-N PLUS PASTE 1. Identification of the substance/mixture and of the company/undertaking 1.1 Product identifier 1.3 Supplier Manufacture Molykote G-N Plus Paste Alfa Laval Tumba AB G.A. Lindberg AB 1.2 Relevant identified uses of the substance or mixture and uses advised against Hans Stahles väg Box 6044 Lubricant SE-147 80 Tumba 164 06 Kista Sweden Sweden Contact details for all countries are continually updated on our website. Please visit www.alfalaval.com to access the information. The latest The latest version of Alfa Laval's SDS is available on our website Tel: +46 8 53 06 50 00 Tel.: +46 8 703 02 00 e-mail: email@example.com Article No 53708601 200 gram 1 of 10 1.4 Emergency telephone number: Dial 112 in case of emergency poisoning and ask for Poison Information both day and night. Dial +46 (0) 8-331231 if you have other questions concerning acute poisonings mon-fri 9.00-17.00 2. Hazards identification 2.1 Classification of the substance or mixture 2.2 Label elements Hazard pictograms - - Signal word - Hazard statements/Risk phrases - Precautionary statements /safety phrases 2.3 Other hazards No information Not hazardous according to article 31 and Annex II of the EU REACH Regulation and its subsequent amendments. This information is based on test data from similar products. S24/25 Avoid contact with skin and eyes S51 Use only in well ventilated areas
P. pastoris has become a versatile expression system for scaling up recombinant protein production6,31,32 and a growing number of protein products are hitting the market33,34. In our previous studies, we cloned the glycoside hydrolase LXYL-P1-2 from L. edodes, expressed the enzyme in the P. pastoris host and performed the preliminary HCDF of engineered yeast28,29,30. Since the enzyme was produced in non-secreted form, all of the cells were harnessed as the biocatalyst to remove xylosyl residual from 7-β-xyloxyl-10-deacetyltaxol (XDT) (and other 7-β-xylosyl-taxanes) in order to form 10-deacetyltaxol (DT), which is the semi-synthetic precursor of Taxol. Using freeze-dried yeast cells harvested from HCDF, we performed the bioconversion of 10 L30. Since the biomass enzyme activity of HCDF was apparently lower than that of flask fermentation, improving this activity would be beneficial to saving a large amount of production costs. Additionally, a great deal of pure oxygen was consumed during HCDF, but its practical application for large-scale fermentation is limited as it is dangerous to handle and is expensive. Therefore, the elimination of a pure oxygen supplement is an economical approach to HCDF, particularly for large-scale fermentation.
To illustrate these differences, we first analyzed bacterial and fungal culture-based microbial communities and simulated datasets. These datasets were then used to validate the performance and taxonomic resolution of both methods. In addition, we investigated the differences in biological interpretation of bacterial and fungal soil- and plant-related microbial communities at family level as analyzed using both the OTU and ASV methods. These soil- and plant-related datasets have higher diversity and are less well identified than the human-related communities that are often used to benchmark metabarcoding workflows [11, 14]. For the soil and plant communities, the effect of non-inversion vs. conventional tillage and rhizosphere vs. endosphere were analyzed, respectively.
The bacterial community was isolated from samples of Belgian field soil where non-inversion and conventional tillage were applied . These communities are referred to below as the soil dataset. This dataset was studied by applying either ASV, usASV, or OTU methods. The unfiltered ASV method supplied fewer ASVs than OTUs, although the difference was rather limited (11,492 ASVs vs. 13,407 OTUs); in contrast, the usASV method was characterized by 21,282 usASVs (Table S2). After application of technical filtering to remove spurious sequences (fewer than two counts in at least three independent samples), a decrease of approximately 70% in the number of ASVs detected for both the ASV and usASV methods. This was mainly due to the removal of unique sample sequences, in contrast to the number of OTUs (decrease of 15%) (Table S2). 2b1af7f3a8